Seyed Hamed Mousavi Alamdardehi; Zarbakht Ansari Pirsaraei; Essa Dirandeh; Mohammad Kazemi Fard
Volume 23, Issue 4 , January 2022, , Pages 525-533
Abstract
Effect of Basil powder and extract on yield and carcass characteristics, relative expression of heat shock protein gene (HSP70) and some blood parameters of broiler chickens under heat stress using 160 Ross 308 one-day-old broiler chicks in a completely randomized design with 5 treatments and 4 replications ...
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Effect of Basil powder and extract on yield and carcass characteristics, relative expression of heat shock protein gene (HSP70) and some blood parameters of broiler chickens under heat stress using 160 Ross 308 one-day-old broiler chicks in a completely randomized design with 5 treatments and 4 replications (8 chickens per replication), was evaluated for 42 days. Treatments included: 1- Basic diet (control), 2- Basic diet+ Vitamin C (250 mg per kg of grain), 3- Basic diet + 25 mg of Basil powder (per kg of grain), 4- Basic diet +50 mg of Basil powder (per kg of grain), 5- The use of Basil extract (1 ml/liter water). Feed intake, body weight gain, feed conversion ratio, carcass characteristics, blood parameters and relative expression of HSP70 gene in blood were measured. The effects of treatments on blood parameters and carcass characteristics were not significant. Relative expression of the HSP70 gene in the blood was significantly reduced in all treatments compared to the control (P<0.05). Based on the results, basil powder and extract reduce the negative effects of heat stress in broilers, so their use in broiler diets is recommended to prevent heat stress.
hamid reza salari; Yousef Jafari Ahangari; Zarbakht Ansari Pirsaraie
Volume 23, Issue 2 , July 2021, , Pages 293-302
Abstract
In this research, the effect of feeding coQ10 and omega 3 fatty acids on testicular histology and relative expression of TGF-b4 & StAR genes in Hubbard roosters was investigated. Forty-eight roosters, at fifty weeks age, were randomly divided into four groups and were subjected to the basal diet ...
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In this research, the effect of feeding coQ10 and omega 3 fatty acids on testicular histology and relative expression of TGF-b4 & StAR genes in Hubbard roosters was investigated. Forty-eight roosters, at fifty weeks age, were randomly divided into four groups and were subjected to the basal diet supplemented with omega-3 (30gr), coQ10 (400mg) and omega-3+ coQ10 (30gr+400mg) per day for eight successive weeks. At the end of the trial, all birds were killed and two samples were collected from the same testicle one of which was processed for histology, whereas another was snap-frozen in liquid nitrogen to assess relative gene expression. Results showed that seminiferous tubule diameters of experimental groups were significantly improved compared to the control group (P<0.05). However, leydig cell numbers and sertoli cells were not significantly affected. The relative expression of TGF-b4 genes were significantly decreased in three groups compared to control (P<0.05). Birds fed coQ10 and omega-3 had significantly higher StAR transcript level compared to other groups (P<0.05). Results of the present study showed that addition of a mixture of coQ10 and omega-3 fatty acids to rooster`s feed, lead to improvement in testicular histology and genes expression related to spermatogenesis.
Michael Gholipour; Vahid Vahedi; Zarbakht Ansari Pirsarei
Volume 22, Issue 3 , September 2020, , Pages 431-440
Abstract
This study was conducted to determine the effects of Propolis on growth performance, carcass characteristics and some blood parameters in broiler chickens in response to heat stress condition. A total of 160 one day-old male broiler chicks (Ross 308) were randomly divided into four treatment groups in ...
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This study was conducted to determine the effects of Propolis on growth performance, carcass characteristics and some blood parameters in broiler chickens in response to heat stress condition. A total of 160 one day-old male broiler chicks (Ross 308) were randomly divided into four treatment groups in a completely randomized design with four replicates of 10 birds each. The experimental treatments including: 1) control (basal diet), 2) the basal diet with Neomycin (200 g/ton), 3) 150 mg/kg Propolis, 4) 200 mg/kg Propolis in diet. Birds submitted to heat stress (32±2ºC from 10.00 AM to 6.00 PM) from 15 to 42 d of age. The results showed that, average mortality was lower in Propolis groups than in the control group (p < 0.05). None of the feed intake data was influenced by treatments during different periods. Birds receiving diet supplemented with 200 mg/kg Propolis had a significantly (p < 0.05) higher body weight compared to the control group at the finisher feeding phase and whole period. In the finisher phase and whole period, the lowest (p < 0.05) fed conversion ratio (FCR) was observed in chickens fed 200 mg/kg Propolis. Cholesterol and LDL level were lower in chickens fed diet with Propolis compared to control (p < 0.05). In conclusion, the addition of Propolis at a rate of 200 mg/kg to the diet could reduce the negative effects of heat stress in broiler chickens.
Amin Kazemizadeh; Ahmad Zare Shahneh; Ali Reza Yousefi; Hasan Mehrabani Yeganeh; Zarbakht Ansari Pirsaraei
Volume 20, Issue 3 , November 2018, , Pages 487-498
Abstract
The present study was conducted to investigate the effect of dietary Curcumin supplementation on histological parameters of testis in aged Ross 308 broiler breeder roosters. A total of twelve 48-week old broiler breeder roosters in a completely randomized design were randomly assigned to four treatments ...
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The present study was conducted to investigate the effect of dietary Curcumin supplementation on histological parameters of testis in aged Ross 308 broiler breeder roosters. A total of twelve 48-week old broiler breeder roosters in a completely randomized design were randomly assigned to four treatments and three replicates during a 13 weeks of experimental period. Treatments included no dietary Curcumin supplementation (control group), and daily supplementation of 10, 20, and 30 mg Curcumin/birds as mixed in the basal diet. At the end of the experimental period, all of the roosters were slaughtered, and testis tissue samples were collected. Testicular weight was higher in the roosters that daily received 30 mg Curcumin compared with the control group (P<0.05). Dietary supplementation of 20 and 30 mg Curcumin/day increased the diameter of seminiferous tubule compared to the control group (P<0.05). Seminiferous epithelium thickness was dose-dependently increased in Curcumin-supplemented birds compared to the control group (P<0.05). The number of spermatogonia cells was increased in all treatment groups compared to the control group (P<0.05). The number of Leydig cells was also increased in roosters received 20 and 20 mg Curcumin/day compared to the control birds (P<0.05). However, treatments did not affect the number of testis blood vessels (P>0.05). According to the results of the present study, dietary supplementation of 30 mg Curcumin/day/bird improves testis histological parameters in aged broiler breeder roosters.